A tool used to estimate the unconjugated bilirubin concentration in blood serum often employs the total bilirubin and direct bilirubin values, typically obtained through laboratory testing. For example, if the total bilirubin is 10 mg/dL and the direct bilirubin is 2 mg/dL, the difference (8 mg/dL) represents the estimated unconjugated fraction.
This estimation provides valuable clinical information in assessing liver function and diagnosing various medical conditions, including hemolytic anemia and Gilbert’s syndrome. Historically, the ability to differentiate between conjugated and unconjugated forms has been essential in understanding bilirubin metabolism and its associated pathologies. This calculated value helps healthcare professionals distinguish between disorders affecting bilirubin conjugation and those affecting its excretion.